Paula Gilbard represented the Animal Justice Party Queensland in the Callide By-election on 18 June 2022. The traditionally conservative electoral district includes the towns of Biloela, Gin Gin and Chinchilla and has an economy that is overly reliant on 'beef' and coal. Gilbard ran on a platform of stopping violent crime, protecting the local environment and diversifying into kinder and more sustainable industries. At the close of voting, the AJP had scored 1.7% of first preferences.

Gilbard said the party went into the campaign with an open mind. "It is the first time the Animal Justice Party has run in the area and we didn't know what to expect. We're happy with the result - it shows that even in areas where many livelihoods rely on animal exploitation there are people itching for change."

Lindon Cox the State Convenor said the campaign was a proof of concept that the Animal Justice Party was not limited to urban districts. "The fact is that there are many people in districts like Callide who agree with the AJP platform, who want to see a better, kinder world for animals and the planet. This is a positive, inclusive movement that will bring new jobs, new opportunities, and a new relationship with nature. I'm proud Paula and the AJP gave people in Callide a choice."

The Animal Justice Party plans to run in many more regional districts in the future.